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ELISAtools

ELISA Data Analysis with Batch Correction

To run data analysis for enzyme-link immunosorbent assays (ELISAs). Either the five- or four-parameter logistic model will be fitted for data of single ELISA. Moreover, the batch effect correction/normalization will be carried out, when there are more than one batches of ELISAs. Feng (2018) <doi:10.1101/483800>.

Functions (29)

ELISAtools-package

ELISA data analysis with batch correction

annotate.plate

S3 method to annotate ELISA plate

Get rid of zeros in a numeric vector

combineData

Combine elisa_batch data

elisa_batch

Constructor function to build an elisa_batch object

elisa_batch-class

S4 class definition of an elisa_batch object

elisa_plate

Constructor function to build an elisa_plate object

elisa_plate-class

S4 class definition of an elisa_plate object

Constructor function to build an elisa_run object

elisa_run-class

S4 class definition of an elisa_run object

f5pl

The five-parameter logistic function

The inverse of the 5-parameter logistic function

Function to load OD data

Read the saved elisa_batch data

Read data according to the design file

plotAlignData

Plot all batch data together

plotBatchData

Plot ELISA data for one batch

Predict the concentration of samples based on fitting

prepareInitsLM

Prepare initial values for fitting shifts

prepareRegInput

Prepare the input for regressoin

Get the OD ranges (min/max)

read.annotation

Read the annotation of single ELISA plate

read.annotations

Read the annotations of plates

Read the single ELISA OD plate

read.plates

Read the ELISA OD files

Report ELISA data in HTML format.

Fit 5- or 4-parameter logistic function

Save the elisa_batch data

saveDataText

Save elisa_batch analysis results

ELISAtools

ELISA Data Analysis with Batch Correction

v0.1.5

MIT + file LICENSE

Authors

Feng Feng [aut, cre]

Initial release

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