amplification efficiencies and experimental Cq1 (optional column)
Fold-amplification within each PCR cycle for each qRT-PCR assay. Determined by qPCR of serial dilutions of the template (see Pfaffl 2001, Nucleic Acids Res 29:00)
data(amp.eff)
A data frame with 17 observations on the following 3 variables.
genea factor with levels actin adk c3 chrom clect eif3h g3pdh gsp2 hsp16 hsp60 hsp90 nd5 r18s rpl11 spon2 tgoln ubl3
efficiencya numeric vector
Cq1a numeric vector
data(amp.eff)
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