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clean

Remove sequences with ambiguous nucleotides from short read classes


Description

Short reads may contain ambiguous base calls (i.e., IUPAC symbols different from A, T, G, C). This generic removes all sequences containing 1 or more ambiguous bases.

Usage

clean(object, ...)

Arguments

object

An object for which clean methods exist; see below to discover these methods.

...

Additional arguments, perhaps used by methods.

Details

The following method is defined, in addition to methods described in class-specific documentation:

clean

signature(x = "DNAStringSet"): Remove all sequences containing non-base (A, C, G, T) IUPAC symbols.

Value

An instance of class(object), containing only sequences with non-redundant nucleotides.

Author(s)

Martin Morgan <mtmorgan@fhcrc.org>

Examples

showMethods('clean')

ShortRead

FASTQ input and manipulation

v1.48.0
Artistic-2.0
Authors
Martin Morgan, Michael Lawrence, Simon Anders
Initial release

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