Signac: AddMotifs – R documentation

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AddMotifs

Add DNA sequence motif information

Description

Construct a Motif object containing DNA sequence motif information and add it to an existing Seurat object or ChromatinAssay. If running on a Seurat object, AddMotifs will also run RegionStats to compute the GC content of each peak and store the results in the feature metadata.

Usage

AddMotifs(object, ...)

## Default S3 method:
AddMotifs(object, genome, pfm, verbose = TRUE, ...)

## S3 method for class 'ChromatinAssay'
AddMotifs(object, genome, pfm, verbose = TRUE, ...)

## S3 method for class 'Seurat'
AddMotifs(object, genome, pfm, assay = NULL, verbose = TRUE, ...)

Arguments

`object`	A Seurat object or ChromatinAssay object
`...`	Additional arguments passed to other methods
`genome`	A `BSgenome` object
`pfm`	A `PFMatrixList` object
`verbose`	Display messages
`assay`	Name of assay to use. If NULL, use the default assay

Value

When running on a ChromatinAssay or Seurat object, returns a modified version of the input object. When running on a matrix, returns a Motif object.

Signac

Analysis of Single-Cell Chromatin Data

v1.2.1

MIT + file LICENSE

Authors

Tim Stuart [aut, cre] (<https://orcid.org/0000-0002-3044-0897>), Avi Srivastava [aut] (<https://orcid.org/0000-0001-9798-2079>), Paul Hoffman [ctb] (<https://orcid.org/0000-0002-7693-8957>), Rahul Satija [ctb] (<https://orcid.org/0000-0001-9448-8833>)

Initial release

2021-05-11