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geNorm

Ranks genes


Description

Uses the geNorm algorithm to determine the most stably expressed genes.

Usage

geNorm(expression, genes = data.frame(Genes = character(0), Avg.M =
  numeric(0)), ctVal = TRUE)

Arguments

expression

a matrix of expression levels. Each row corresponds to a sample and each column to a gene.

genes

a data frame to output the result of the function

ctVal

a logical value indicating data type. If ct-values are input, ctVal=TRUE, otherwise, ctVal=FALSE.

Value

A sorted dataframe with two columns, 'Genes' and 'Avg.M'. The last two genes are the two most stable control genes.

Avg.M is average expression stability values (M) of remaining control genes during stepwise exclusion of the least stable control gene.

References

Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F (2002) <doi: 10.1186/gb-2002-3-7-research0034>.

Examples

FIB
geNorm(FIB,ctVal=FALSE)
FIBct
geNorm(FIBct)

ctrlGene

Assess the Stability of Candidate Housekeeping Genes

v1.0.1
GPL (>= 2)
Authors
Shanliang Zhong [aut, cre]
Initial release

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