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read.marrayLayout

Create objects of class marrayLayout


Description

This function creates objects of class marrayLayout to store layout parameters for two-color cDNA microarrays.

Usage

read.marrayLayout(fname = NULL, ngr, ngc, nsr, nsc, pl.col = NULL, ctl.col = NULL, sub.col = NULL, notes = fname, skip, sep = "\t", quote = "\"", ...)

Arguments

fname

the name of the file that stores plate and control information. This is usually a file obtained from a database.

ngr

the number of rows of grids per image.

ngc

the number of columns of grids per image.

nsr

the number of rows of spots per grid.

nsc

the number of columns of spots per grid.

pl.col

the column number in fname that contains plate information.

ctl.col

the column number in fname that contains control information.

sub.col

the column number in fname that contains full ID information.

notes

object of class character, vector of explanatory text.

skip

the number of lines of the data file to skip before beginning to read data.

sep

the field separator character. Values on each line of the file are separated by this character. The default is to read a tab delimited file.

quote

the set of quoting characters. By default, this is disable by setting ‘quote="\""’.

...

further arguments to scan.

Value

An object of class marrayLayout.

Author(s)

References

http://www.bioconductor.org/

Examples

datadir <- system.file("swirldata", package="marray")

### Reading in control information from file
skip <-  grep("Row", readLines(file.path(datadir,"fish.gal"), n=100)) - 1
swirl.layout <- read.marrayLayout(fname=file.path(datadir,"fish.gal"), ngr=4, ngc=4,
nsr=22, nsc=24, ctl.col=4, skip=skip)

### Setting control information.
swirl.gnames <- read.marrayInfo(file.path(datadir,"fish.gal"), info.id=4:5, labels=5, skip=21)
x <-  maInfo(swirl.gnames)[,1]
y <- rep(0, maNspots(swirl.layout))
y[x == "control"] <- 1
slot(swirl.layout, "maControls") <- as.factor(y)

marray

Exploratory analysis for two-color spotted microarray data

v1.68.0
LGPL
Authors
Yee Hwa (Jean) Yang <jeany@maths.usyd.edu.au> with contributions from Agnes Paquet and Sandrine Dudoit.
Initial release
2009-08-15

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